Chapter 20 DNA Technology p. 375

• Genetic engineering
• Biotechnology

DNA Cloning p. 375

• Gene cloning

 

DNA technology makes it possible to clone genes for basic research and commercial application p. 376

• plasmids
• Recombinant DNA
• What are potential uses for cloned DNA?
• Figure 20.1 p. 376

Restriction enzymes are used to make recombinant  p. 376

• What are 3 “biological tools” needed for genetic engineering?
• Restriction
• Restriction Enzymes
• Restriction fragments
• Sticky ends
• DNA ligase
• Ampicillin
• Figure 20.2 p. 377

Genes can be cloned in recombinant DNA vectors p. 378

• Vectors(cloning vectors)
• What cells are the most popular types of vectors?

           

Procedure for cloning a eukaryotic gene in a bacterial plasmid p. 378

• Figure 20.3 p. 378
• What are the five steps? You should have an awareness of how this process works.  Figure 19.3 p. 373 is helpful in understanding this process.

 

• Nucleic acid hybridization
• Nucleic acid probe

 

The polymerase chain reaction (PCR) clones DNA entirely in vitro p. 382

• Polymerase chain reaction (PCR)
• Figure 20.7 p. 382

DNA Analysis and Genomics p. 383

• Genomics
• Gel electrophoresis

 

Restriction fragments analysis detects DNA differences that affect restriction sites p. 383

• Steps for Using Bacteria and Plasmids to clone genes.
• Figure 20.8 p. 384
• Figure 20.9 p. 384
• Figure 20.10 p. 385

Human Gene Therapy p. 393

• Gene therapy

 

Pharmaceutical Products p. 395

• Vaccine
• DNA fingerprint